Protein quantification analysis-Label-free (Serum)
To avoid this result, we provide the label-free analysis service to solve your problem.
We suggest using label-free analysis to quantify proteins in serum sample.
Here's the protein identify numbers compared between i-TRAQ and label-free analysis.
| Basic analysis | Standard analysis | Advance analysis | |
| i-TRAQ analysis (serum sample) | ~100 | 80-150 | 200-300 |
| Label-free (serum sample) | ~200 | 160-300 | 800~1200 |
For Serum sample, we recommend you to use label-free analysis for deeper protein information.
Working day not including delivery time: 3-4 weeks
Plasma abundant proteins (Top-12) removal
(a) Sample type: Human body fluids (serum, plasma, urine, interstitial fluid)
(b) Sample amount: ≥50 uL
(c) Sample concentration: ≥40 ug/uL
*Top-12 abundant proteins list
| α1-Acid Glycoprotein |
| α1-Antitrypsin |
| α2-Macroglubulin |
| Albumin |
| Apolipoprotein A-I |
| Apolipoprotein A-II |
| Fibrinogen |
| Haptoglobin |
| IgA |
| IgG |
| IgM |
| Transferrin |
In-gel protein identification: Standard/Advanced analysis
(a) Sample type: Cell pellet, Protein extracts
(b) Sample volume: ≤50 uL
(c) Sample concentration: ≥0.2 ug/uL (silver stain), ≥1 ug/uL (Coomassie blue stain)
(d) Platform: Hoefer SE260, 1DLC, LTQ-Orbitrap MS
Please click the link below for the specification of Proteomics service
Sample requirement
1. Summary report. pdf
2. Protein-peptide report (Identification result and Raw ratio). xlsx
3. Normalized Protein Ratio (Global normalization). xlsx